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goat anti rabbit immunoglobulin gamma secondary antibody (OriGene)

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OriGene goat anti rabbit immunoglobulin gamma secondary antibody
Goat Anti Rabbit Immunoglobulin Gamma Secondary Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+igg/Rabbit+IgG+(F(ab')2+specific%2C+adsorbed)+Goat+Polyclonal+Antibody/pm41511720-76-14-21
Average 92 stars, based on 50 article reviews

goat anti rabbit immunoglobulin gamma secondary antibody - by Bioz Stars, 2026-09

92/100 stars

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Blocking Assay:

Article Title: Gastrodin regulates expression of angiotensin-SirT3 and proinflammatory mediators in reactive astrocytes via activated microglia
Article Snippet: .. Blocking was with skim milk for 1-2h.The membranes were incubated with β-actin (Mouse monoclonal IgG 1:5000; Sigma;), C3(rabbit monoclonal IgG 1:1000; abcam), S100A10 (mouse monoclonal IgG 1:1000;Thermo Fisher), AT1 (rabbit polyclonal IgG 1:500; abcam), ATO (rabbit polyclonal IgG 1:1000; ORIGENE), TNF-α (rabbit polyclonal IgG 1:1000; Milipore), SirT3 (rabbit polyclonal IgG 1:500; Santa Cruz Biotechnology), IGF-1(rabbit polyclonal antibody 1:1000;ORIGENE ), BDNF(rabbit polyclonal antibody 1:1000 ORIGENE) primary antibodies diluted in blocking solution overnight at 4 ℃. .. Following incubation, the membranes were removed and rinsed quickly three times; secondary antibodies (Goat anti-Mouse IgG, Peroxidase Conjugated; 1: 5000; Milipore; USA, Goat anti-Rabbit IgG, Peroxidase Conjugated; 1: 5000; Milipore; USA) were then added and incubated at room temperature 1h.

Incubation:

Article Title: Gastrodin regulates the expression of renin-angiotensin system-SIRT3 and proinflammatory mediators in reactive astrocytes via activated microglia.
Article Snippet: Edited by: John Foxe Abstract Gastrodin, an anti-inflammatory herbal agent, is known to suppress microglia activation.. Here, we investigated whether it would exert a similar effect in reactive astrocytes and whether it might act through the renin-angiotensin system (RAS) and sirtuin 3 (SIRT3).. Angiotensinogen (ATO), angiotensinconverting enzyme (ACE), angiotensin II type 1 (AT1) and type 2 (AT2) receptor and SIRT3 expression was detected in TNC-1 astrocytes treated with BV-2 microglia conditioned medium (CM) with or without gastrodin and lipopolysaccharide (LPS) pre-treatment by RT-PCR, immunofluorescence and western blotting analysis.

Membrane:

Article Title: Adaptive double-saturated control for hovering over an asteroid
Article Snippet: The role of perfluorodecanoic acid (PFDA) in gastric carcinogenesis and its mechanism remains unknown.. Our previous research revealed that PFDA regulated the growth of human gastric cells.. However, its core molecules and basic mechanisms are still not clear.

Staining:

Article Title: Is low molecular weight hyaluronan an early indicator of disease in avian systemic sclerosis?
Article Snippet: Ac ce pte d M 1 Aim of the study: To further elucidate the pathogenesis of systemic sclerosis (SSc) an experimental avian model was used.. University of California at Davies line 200-chicken (UCD200) spontaneously develops a SSc like disease that has most features of human SSc with vascular effects, inflammation, autoimmunity and fibrosis.. The first signs of disease in UCD-200 are swelling and ischemic lesions of the comb, a tissue containing high amounts of the glycosaminoglycan hyaluronan.

Article Title: Effects of non-cytotoxic drugs on the growth of multidrug-resistance human gastric carcinoma cell line.
Article Snippet: .. Immunocytochemical staining was performed with rabbit polyclonal IgG specific for PCNA (Zhongshan Biotechnology, Beijing, China) in a dilution of 1:200 over night at 4°C, and then reacted with corresponding horseradish peroxiodase (HRP)-conjugated anti-IgG as secondary antibody. ..